CNE (Cationic Nano Emulsion) Vaccine Adjuvants are the oil-in-water Cationic Nano Emulsion made of squalene or mineral oil droplets and 1-2 mg of cationic lipid DOTAP (1,2-Di-(9Z-octadecenoyl)-3-trimethylammoniumpropane methyl sulfate).

• CAS 40: cationic oil-in-water nanoemulsion oil with particle size of 40 nm. Squalene and DOTAP
• CAS 100: cationic oil-in-water nanoemulsion oil with particle size of 100 nm. Squalene and DOTAP
• CAS 150: cationic oil-in-water nanoemulsion oil with particle size of 150 nm. Squalene and DOTAP
• CAS 40M: cationic oil-in-water nanoemulsion oil with particle size of 40 nm. Mineral oil and DOTAP
• CAS 100M: cationic oil-in-water nanoemulsion oil with particle size of 100 nm. Mineral oil and DOTAP
• CAS 150M: cationic oil-in-water nanoemulsion oil with particle size of 150 nm. Mineral oil and DOTAP

The cationic component DOTAP makes squalene or mineral oil based nano-emulsions the CNE adjuvants that allow the association with mRNA & plasmid DNA to form an efficient nanoparticle delivery system. SDA BIO CNE (CAS) are non-viral gene delivery systems, self-assembled from cationic entities and negatively charged immunogen that function as vaccine carrier.

CNE Vaccine Adjuvant is biodegradable squalene for human use and veterinary adjuvants contains mineral oil (Drakeol 6VR).

CNE induces local stimulation and recruitment of DCs and granulocytes, differentiation of monocytes into DCs and increased uptake of antigen by DCs. The emulsion acts more specifically on macrophages present at the site of injection. A local increase of chemokines released also influences the recruitment of immune cells from the blood to the site of vaccination, creating an amplification loop. These formulations enhance differentiation of monocytes towards a mature phenotype, thereby promoting migration of antigen-loaded cells to the draining lymph node.

SDA BIO is known as a supplier of vaccine adjuvants. It has several locations in Research Triangle Park of North Carolina including the manufacturing site in Sanford. SDA BIO is also a service organization in Biotechnology Health Products and MS&T. Key products: self-emulsifying/assembling delivery/adjuvants for biologics, oil-in-water, ~150nm, ~100nm, ~40nm (IMS, microemulsion, or emulsion based immunosol), water-in-oil-in-water, ~300nm, water-in-oil, <1000nm, latex nanoparticle, 80-100nm, lipid nanoparticle, polymer gel nanoparticle. We have multiple partnerships in commercializing soluble immunostimulants and systemic or complex adjuvants. Our squalene based O/W 100~150 nm (AS110 and AS150) and 40 nm (AS40) human vaccine adjuvants are self-emulsifying oil phase, free of microfluidizer or high gear homogenizer. Our lipid nanoparticle delivery system (LNP) and cationic o/w nano-emulsion (CNE) are self-assembling. CAS40 is transparent formulation with particle size ~40 nm; CAS110 is emulsion type of 100 nm in size; CAS150 is emulsion type of 150 nm in size. Our squalene based nanoemulsion is extremely stable, for as long time as > 1 year under as high temperature as 42 degree C. Both emulsion and polymer gel based adjuvants can serve as diluent of live attenuated vaccines. SDA BIO provides contracting services on GMP (federal regulation, quality systems) and MS&T, both 21CFR and 7CFR. To know more of our services and products, please contact us at +1 (919) 349-3023, or by email sdabiocom@gmail.com, we welcome you visit our website sdabio.com, meet us at 800 Office Park Drive, Research Triangle Park, NC 27709 and 331 Goldsboro Avenue, Sanford, NC 27330.