Cationic NanoEmulsion (CNE) Vaccine Adjuvant

SDA BIO provides non-cationic, non-emulsified nanoemulsion squalane oils, NE60 = 60 nm, NE80 = 80 nm, NE100 = 100 nm. These are the oil phases of self-emulsifying nanoemulsions. You add cationic lipids with your desired quantity and quality to generate CNE oil, then combine these CNE oil with RNA/DNA solution by gentle mixing, at ratio of 1:4-1:8 to result final vaccines

Illustration of an oil-in-water cationic nanoemulsion, showing surfactants and cationic DOTAP lipids in a spherical arrangement.
Diagram illustrating anionic oil-in-water nanoemulsion, highlighting DOTAP and ALC-0315 along with a representation of cell membrane interactions.
Illustration of an oil-in-water cationic nanoemulsion interacting with a cell, showing charged particles and the delivery approach.
Illustration of three immunoenhancer systems featuring a molecular structure labeled 'Saponin', a spherical structure labeled 'CpG', and a molecular structure labeled 'DDA'.

CNE (Cationic Nano Emulsion) Vaccine Adjuvants are the oil-in-water Cationic Nano Emulsion made of squalene or mineral oil droplets and 1 mg of cationic lipid DOTAP (1,2-Di-(9Z-octadecenoyl)-3-trimethylammoniumpropane methyl sulfate).

  • CAS 60: cationic oil-in-water nanoemulsion oil with particle size of 60 nm. Squalene and DOTAP
  • CAS 80: cationic oil-in-water nanoemulsion oil with particle size of 80 nm. Squalene and DOTAP
  • CAS 100: cationic oil-in-water nanoemulsion oil with particle size of 100 nm. Squalene and DOTAP
  • CAS 60M: cationic oil-in-water nanoemulsion oil with particle size of 60 nm. Mineral oil and DOTAP
  • CAS 80M: cationic oil-in-water nanoemulsion oil with particle size of 80 nm. Mineral oil and DOTAP
  • CAS 100M: cationic oil-in-water nanoemulsion oil with particle size of 100 nm. Mineral oil and DOTAP

The cationic component DOTAP leads to squalene or mineral oil based nano-emulsions positively charged on the oil drops, the CNE adjuvants that allow the association with mRNA & plasmid DNA to form an efficient nanoparticle delivery system. SDA BIO CNE (CAS) are non-viral gene delivery systems. Once mixed with aqueous nucleic “antigen generator”, the self-assembled cationic entities and negatively charged immunogen combine to function as nucleic vaccine.

CNE Vaccine Adjuvant is USP grade squalene for human use and veterinary adjuvants contains mineral oil.

CAS100:

  • Cationic Oil-in-Water Emulsion, 600 mg squalene, 20 mg DOTAP, with particle size of 100 nm without nucleic acid.
  • Ready to combine with aqueous antigen at ratio of 1 part of CAS100 to 4-9 parts of samRNA antigen
  • Viscosity: <20 mPa.s

CAS80:

  • Cationic Oil-in-Water Emulsion, 300 mg squalene, 20 mg DOTAP, with particle size of 80 nm without nucleic acid.
  • Ready to combine with aqueous antigen at ratio of 1 part of CAS80 to 4-9 parts of samRNA antigen
  • Viscosity: <20 mPa.s

CAS60:

  • Cationic Oil-in-Water Emulsion, 300 mg squalene, 20 mg DOTAP, with particle size of 60 nm without nucleic acid, 80-100 nm with samRNA
  • Ready to combine with aqueous antigen at ratio of 1 part of CAS60 to 4-9 parts of samRNA antigen
  • Viscosity: <20 mPa.s

Global Professionals Come to Visit Us at Research Triangle Park, North Carolina

SDA BIO US Manufacturing Site 331 Goldsboro Avenue Sanford NC 27330